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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
18/04/2022 |
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Data da última atualização: |
18/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FLORENCIO, C.; BADINO, A. C.; FARINAS, C. S. |
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Afiliação: |
CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Addition of soybean protein improves saccharification and ethanol production from hydrothermally pretreated sugarcane bagasse. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BioEnergy Research, v. 12, n. 1, 2019. |
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Páginas: |
81?93 |
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DOI: |
https://doi.org/10.1007/s12155-018-9956-6 |
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Idioma: |
Inglês |
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Conteúdo: |
The bioconversion yield of ethanol from lignocellulosic feedstocks is negatively affected by the unproductive adsorption of cellulolytic enzymes onto lignin. In this work, soybean protein was used as a lignin-blocking additive, with the aim of improving the production of ethanol from enzymatic hydrolysates of pretreated sugarcane bagasse. Investigation was made of the effects of the type of hydrothermal pretreatment process?steam explosion (SE) or liquid hot water (LHW), loadings of solids and enzymes, and bioreactor type. The addition of soybean protein led to a exceptional 76% increase of glucose released using the LHW pretreated bagasse, after 24 h of reaction, employing a high-solids loading (15%, w/v) and a low enzyme dosage (5 FPU/g dry biomass). A significant improvement was also achieved for industrial-like mixing conditions in a bench-scale stirred tank reactor, increasing the glucose released by 61 and 42% for the LHW and SE processes, respectively. Ethanol production was also positively affected by the presence of soybean protein, with increases of up to 86 and 65% for the LHW and SE hydrolysates, compared to the control experiment. Characterization of the sugarcane bagasse after the adsorption of soybean protein, using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), corroborated the higher affinity of the additive for the LHW bagasse. These findings suggest that soybean protein supplementation during enzymatic hydrolysis by commercially available enzymes is an effective strategy for achieving higher saccharification yields from hydrothermally pretreated biomass, hence improving ethanol production MenosThe bioconversion yield of ethanol from lignocellulosic feedstocks is negatively affected by the unproductive adsorption of cellulolytic enzymes onto lignin. In this work, soybean protein was used as a lignin-blocking additive, with the aim of improving the production of ethanol from enzymatic hydrolysates of pretreated sugarcane bagasse. Investigation was made of the effects of the type of hydrothermal pretreatment process?steam explosion (SE) or liquid hot water (LHW), loadings of solids and enzymes, and bioreactor type. The addition of soybean protein led to a exceptional 76% increase of glucose released using the LHW pretreated bagasse, after 24 h of reaction, employing a high-solids loading (15%, w/v) and a low enzyme dosage (5 FPU/g dry biomass). A significant improvement was also achieved for industrial-like mixing conditions in a bench-scale stirred tank reactor, increasing the glucose released by 61 and 42% for the LHW and SE processes, respectively. Ethanol production was also positively affected by the presence of soybean protein, with increases of up to 86 and 65% for the LHW and SE hydrolysates, compared to the control experiment. Characterization of the sugarcane bagasse after the adsorption of soybean protein, using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), corroborated the higher affinity of the additive for the LHW bagasse. These findings suggest that soybean protein supplementation during enzymatic hydrolysis by ... Mostrar Tudo |
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Palavras-Chave: |
Lignocellulosic biomass; Unproductive adsorption. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142272/1/P-Addition-of-Soybean-Protein-Improves-Saccharification-and-Ethanol.pdf
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Marc: |
LEADER 02311naa a2200193 a 4500
001 2142272
005 2022-04-18
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12155-018-9956-6$2DOI
100 1 $aFLORENCIO, C.
245 $aAddition of soybean protein improves saccharification and ethanol production from hydrothermally pretreated sugarcane bagasse.$h[electronic resource]
260 $c2022
300 $a81?93
520 $aThe bioconversion yield of ethanol from lignocellulosic feedstocks is negatively affected by the unproductive adsorption of cellulolytic enzymes onto lignin. In this work, soybean protein was used as a lignin-blocking additive, with the aim of improving the production of ethanol from enzymatic hydrolysates of pretreated sugarcane bagasse. Investigation was made of the effects of the type of hydrothermal pretreatment process?steam explosion (SE) or liquid hot water (LHW), loadings of solids and enzymes, and bioreactor type. The addition of soybean protein led to a exceptional 76% increase of glucose released using the LHW pretreated bagasse, after 24 h of reaction, employing a high-solids loading (15%, w/v) and a low enzyme dosage (5 FPU/g dry biomass). A significant improvement was also achieved for industrial-like mixing conditions in a bench-scale stirred tank reactor, increasing the glucose released by 61 and 42% for the LHW and SE processes, respectively. Ethanol production was also positively affected by the presence of soybean protein, with increases of up to 86 and 65% for the LHW and SE hydrolysates, compared to the control experiment. Characterization of the sugarcane bagasse after the adsorption of soybean protein, using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), corroborated the higher affinity of the additive for the LHW bagasse. These findings suggest that soybean protein supplementation during enzymatic hydrolysis by commercially available enzymes is an effective strategy for achieving higher saccharification yields from hydrothermally pretreated biomass, hence improving ethanol production
653 $aLignocellulosic biomass
653 $aUnproductive adsorption
700 1 $aBADINO, A. C.
700 1 $aFARINAS, C. S.
773 $tBioEnergy Research$gv. 12, n. 1, 2019.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 45 | |
| 10. |  | NAGANO, L. F. P.; FLORENCIO, C.; FARINAS, C. S. Inclusão do esterco bovino ao conceito de biorrefinarias: avaliação no uso como substrato para produção de enzimas hemi(celulolíticas) In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 4., 2012, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2012. p. 22. Editores técnicos: João de Mendonça Naime, Lucimara Aparecida Forato, Maria Alice Martins, Ladislau Marcelino Rabello, Rubens Bernardes Filho. (Embrapa Instrumentação. Documentos, 56).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | DUTRA, J. F.; FLORENCIO, C.; FARINAS, C. S. Potencial biotecnológico de fungos isolados de área de mineração em relação à produção de enzimas de interesse agroindustrial. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2017. p. 20. Editores técnicos: Alexandre Berndt, Ana Rita de Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos. (Embrapa Pecuária Sudeste, Documentos, 126).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | GAVA JUNIOR, M.; FLORENCIO, C.; KLAIC, R.; FARINAS, C. S. Produção de ácidos orgânicos por duas linhagens de fungos filamentosos por fermentação em estado sólido. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 9., 2017, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2017. p. 30. Editores técnicos: Alexandre Berndt, Ana Rita de Araujo Nogueira, Bianca Baccili Zanotto Vigna, Juliana Gonçalves Costa, Lea Chapaval, Manuel Antonio Chagas Jacinto, Patricia Menezes Santos. (Embrapa Pecuária Sudeste, Documentos, 126).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | FLORENCIO, C.; BADINO JUNIOR, A. C.; FARINAS, C. S. Efeito de aditivos na hidrólise enzimática do bagaço de cana-de-açúcar In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 8., 2016, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2016. p.122. Editores técnicos: Wilson Tadeu Lopes da Silva, José Manoel Marconcini, Maria Alice Martins, Lucimara Aparecida Forato, Paulino Ribeiro Villas Boas. (Embrapa Instrumentação. Documentos, 61).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 20. | | BERTAZZO, G. B.; OLDONI, F. C. A.; FLORENCIO, C.; BOGUSZ JUNIOR, S.; FERREIRA, M. D. Avaliação de compostos voláteis em mamão (Carica papaya L). In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 12., 2020, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação: Embrapa Pecuária Sudeste, 2020. Editores técnicos: Cristiane Sanchez Farinas, Daniel Souza Corrêa, José Manoel Marconcini, Maria Fernanda Berlingieri Durigan, Paulo Sérgio de Paula Herrmann Junior. Embrapa Instrumentação. Documentos, 71.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 45 | |
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